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		III. Transplantation of the neural crest 1. Prepare a small pocket in the ventral surface of the host embryo. Only a slit is required by cutting with a glass needle. The pocket can be enlarged to receive the transplant by carefully removing some of the mesoderm cells with the tip of the glass needle. 2. Using a fine-tipped needle, remove part of the neural fold on the right side of the embryo. With a single motion from the anterior to the posterior, use the glass needle to make a thin tear in the pigmented epidermis very close to the outer edge of the right neural fold. Now, tear carefully through the whitish mesodermal cells that lie underneath. When the grayish-tan endodermal cells become visible, stop cutting. Follow the first longitudinal cut with a second parallel cut at the inner edge of the right neural fold. Next, make two transverse cuts, anterior to posterior, to complete a rectangular area of neural fold tissue. 3. Lift the entire rectangular area of neural tissue away from the embryo with the tip of the glass needle. The piece should include a whitish mesoderm with the overlying pigmented ectoderm.