neural crest

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Protocol

I. Preparation of Microsurgery Tools

Follow protocol found on the general protocol web page.

II. Microsurgery preparation:

1. Manually dejelly embryo of stage 14 and keep in HSBt containing antibiotics until use. For microsurgery, also remove membrane around the embryo.

2. Prepare 2% agarose-coated operating dish. Rinse with HBSt and antibiotics.

3. Place embryos into operating dish containing 1X HBSt and antibiotics.

4. Use clean technique while performing microsurgery (i.e. dip all tools, pipettes, and glass bridges into 70% ethanol and then into sterile HBSt before using).

Last Modified: 5 August 2001

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		Protocol I. Preparation of Microsurgery Tools Follow protocol found on the general protocol web page.
		II. Microsurgery preparation: 1. Manually dejelly embryo of stage 14 and keep in HSBt containing antibiotics until use. For microsurgery, also remove membrane around the embryo. 2. Prepare 2% agarose-coated operating dish. Rinse with HBSt and antibiotics. 3. Place embryos into operating dish containing 1X HBSt and antibiotics. 4. Use clean technique while performing microsurgery (i.e. dip all tools, pipettes, and glass bridges into 70% ethanol and then into sterile HBSt before using).