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Instructor's prep sheet for Sea Urchin Fertilization ASW (Artificial sea water, Woods Hole MBL), 5 liters# 0.5 M KCl to induce gamete shedding,100 ml Prep checklist: Paper towels (C-fold) Beakers (approximately the size of the urchins)# aluminum foil 60 mm Petri dishes 20 g needles 1 ml syringes needle disposal container wide-bore pastuer pipets and bulbs or non-sterile transfer pipets depression slides microscope slides coverslips Gloves- small, medium and large (1 box each) test tube racks 100 mm tubes marker labelling tape Finger bowls #   # Beakers, finger bowls, test tubes and the bottles used to make the sea water need to be very clean of soap or other contaminants. Rinse thoroughly before use.