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		Animals We will be using Lytechinus variegatus from Florida. The gametes and embryos can be kept at room temperature. Figure 1. Lytechinus variegatus adults Protocol This experiment will involve the segregation of eggs into four samples. Sample 1 will be the control, exposed to NO radiation. In sample 2, the eggs will be irradiated before fertilization. Sample 3 will be irradiated 10 minutes after fertilization. Sample 4 will be irradiated 30 minutes after fertilization. The experiment is to be carried out as follows. Collection of Gametes Follow procedure given in basic protocol. UV Radiation Irradiate sample #2 (unfertilized eggs) using the UV crosslinker set on the default setting of intensity 120,000 microJoules/cm2 for a time of two minutes. The crosslinker has radiation with a wavelength of 250nm. Fertilization Fertilize all unirradiated samples as one batch, and then split into three separate test tubes following the completion of fertilization. Fertilize the irradiated sample as a separate batch. For fertilization procedure, follow instructions given in basic protocol.