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Results and
Discussion
The
two samples behaved quite differently. The Ig8 staining
revealed that PMCs of the control group had in fact migrated
after gastrulation to form the endoskeleton, or spicules, of
the urchin. In the experimental group, none of the embryos
had undergone complete gastrulation. The Ig8 staining showed
that the PMC cells had failed to migrate out, the embryo
itself had failed to develop.
Our original hope, that we
could block skeleton formation in the urchin, was satisfied
by this experiment. We also observed that
NiCl2disrupts
not only skeleton formation, but the process of gastrulation
as well.
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